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Viral hemorrhagic septicemia virus
Viral hemorrhagic septicemia virus
規(guī)格:
貨期:
編號(hào):B162834
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Viral hemorrhagic septicemia virus
商品貨號(hào) B162834
Classification Rhabdoviridae, Novirhabdovirus, Piscine novirhabdovirus
Agent Viral hemorrhagic septicemia virus
Strain Makah
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1872 images
Comments Derived by adaptation of CHSE (ATCC® CRL-1681™) cell culture product ATCC® VR-1387™ to EPC cells (ATCC® CRL-2872™). ATCC® VR-1387™ and ATCC® VR-1872™ have not been compared for sequence or infectivity.
Effect on Host
CPE, refractile rounding and degeneration
Recommended Host
Epithelioma Papulosum Cyprini (EPC) cells (ATCC® CRL-2872™)
Growth Conditions

Temperature: 15°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 15°C in a humidified atmosphere. End adsorption by adding virus growth medium.

Incubation: 2-4 days at 15°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor J Winton
Source Isolated by R. Brunson from ovarian fluid of normal adult coho salmon returning in 1988 to the Makah National Fishery, WA.
References

Bernard J, et al. Nucleocapsid gene sequence of a North American isolate of viral haemorrhagic septicaemia virus, a fish rhabdovirus. J. Gen. Virol. 73: 1011-1014, 1992. PubMed: 1634868

Winton JR. . Proc. 2nd Int. Symp. Viruses Lower Verts : 43-50, 1991.

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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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