欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > 16-3-1N
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
16-3-1N
16-3-1N
規(guī)格:
貨期:
編號:B163594
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 16-3-1N
商品貨號 B163594
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody cross-reacts with H-2Kq, H-2r and H-2p.
This line was derived from the same fusion used to produce ATCC HB-16 (16-1-11N) and displays the same pattern of reactivity.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
This line was derived from the same fusion used to produce ATCC HB-16 (16-1-11N) and displays the same pattern of reactivity.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 K k; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against H-2 K k; cytotoxic
Comments
Animals were immunized with spleen cells from C3H mice.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with H-2Kq, H-2r and H-2p.
This line was derived from the same fusion used to produce ATCC HB-16 (16-1-11N) and displays the same pattern of reactivity.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a; kappa light chain
Name of Depositor DH Sachs, K Ozato
Deposited As mouse (B cell); mouse (myeloma)
References

Ozato K, et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
浠水县| 临城县| 兴化市| 湘潭县| 吉木乃县| 双流县| 泾阳县| 鱼台县| 庆城县| 卢湾区| 孝义市| 孟州市| 武陟县| 嘉鱼县| 白玉县| 武冈市| 绥棱县| 阿勒泰市| 小金县| 秭归县| 临泉县| 靖西县| 保山市| 城固县| 洛川县| 义乌市| 四川省| 永平县| 都兰县| 班玛县| 平乐县| 行唐县| 嵊州市| 如皋市| 宜春市| 哈密市| 土默特左旗| 桦甸市| 桃江县| 凯里市| 马尔康县|