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B1B3 [464 B1B3]
B1B3 [464 B1B3]
規(guī)格:
貨期:
編號:B163974
品牌:Mingzhoubio

標準菌株
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DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
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產(chǎn)品名稱 B1B3 [464 B1B3]
商品貨號 B163974
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to an epitope in amino acids 3506 through 3635 on human apolipoprotein B.
Derivation
Animals were immunized with human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Clinical Data
Animals were immunized with human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia.
Genes Expressed
immunoglobulin; monoclonal antibody; against human low density lipoprotein
Cellular Products
immunoglobulin; monoclonal antibody; against human low density lipoprotein
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to an epitope in amino acids 3506 through 3635 on human apolipoprotein B.
It cross competes with antibody B1B6 (see ATCC CRL-2248).
The antibody blocks binding of LDL to LDL receptors.
It does not bind apolipoprotein B48.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor G Schonfeld
Deposited As mouse (B cell); mouse (myeloma)
References

Krul ES, et al. Roles of apolipoproteins B and E in the cellular binding of very low density lipoproteins. J. Clin. Invest. 75: 361-369, 1985. PubMed: 3973009

Hahm KS, et al. Limited proteolysis selectively destroys epitopes on apolipoprotein B in low density lipoproteins. J. Lipid Res. 24: 877-885, 1983. PubMed: 6195279

Krul ES, et al. Regional specificities of monoclonal anti-human apolipoprotein B antibodies. J. Lipid Res. 29: 937-947, 1988. PubMed: 2457642

Kinoshita M, et al. Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. J. Lipid Res. 31: 701-708, 1990. PubMed: 1693649

Tikkanen MJ, et al. Expression of apolipoprotein B epitopes in very low density lipoprotein subfractions. Studies with monoclonal antibodies. Arteriosclerosis 4: 138-146, 1984. PubMed: 6200098

Krul ES, et al. Two distinct truncated apolipoprotein B species in a kindred with hypobetalipoproteinemia. Arteriosclerosis 9: 856-868, 1989. PubMed: 2574033

Tikkanen NJ, et al. Antigenic mapping of human low density lipoprotein with monoclonal antibodies. J. Lipid Res. 23: 1032-1038, 1982. PubMed: 6183385

Tikkanen MJ, et al. Differential reactivity of human low density lipoproteins with monoclonal antibodies. J. Lipid Res. 24: 1494-1499, 1983. PubMed: 6197500

Krul ES, et al. Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function. J. Lipid Res. 29: 1309-1325, 1988. PubMed: 2466929

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