欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > BW5147.3
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
BW5147.3
BW5147.3
規(guī)格:
貨期:
編號(hào):B164059
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 BW5147.3
商品貨號(hào) B164059
Organism Mus musculus, mouse
Tissue thymus
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain AKR/J
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a clone of the BW5147 cell line which was established from a spontaneous AKR/J thymoma.
Antigen Expression
H-2k; Thy-1.1
Comments

The cells are HAT resistant but sensitive to 0.001 mM cortisol and to 25 mcg/mL PHA.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Name of Depositor R Hyman, P Ralph
Deposited As Mus musculus
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
保亭| 安溪县| 方山县| 东宁县| 文成县| 苏尼特右旗| 鄂州市| 宜城市| 长垣县| 沐川县| 宁国市| 延津县| 漳州市| 定安县| 萝北县| 铅山县| 二连浩特市| 涿鹿县| 绥滨县| 革吉县| 东安县| 重庆市| 民勤县| 玉树县| 重庆市| 蓬溪县| 大英县| 古蔺县| 涿州市| 江永县| 同仁县| 台北市| 新野县| 锡林浩特市| 土默特右旗| 韶关市| 大城县| 白银市| 疏附县| 汶上县| 泸溪县|