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male

Caucasian

62 years

Cells senesce after approximately 52 population doublings.

Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: 1:2 to 1:6 is recommened

Medium Renewal: Every 2 to 3 days

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phaseliquid nitrogen vapor phase

Temperature: 37.0°C