欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pFL59-
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pFL59-
pFL59-
規(guī)格:
貨期:
編號:B216827
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pFL59-
商品貨號 B216827
Designations pFL59-
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 4.8010001182556150
Vector: pFL59- (phagemid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):TRP1,ampR
Construct size (kb): 4.801000118255615
Features: insert detection: lacZ'
marker(s): ampR, TRP1
promoter: lac
replicon: pMB1, f1, ARS-CEN6
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--4.0, 0.85; ClaI--4.0, 0.8.
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR - f1 ori.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827

Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361

Panzeri L, Philippsen P. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae. EMBO J. 1: 1605-1611, 1982. PubMed: 6327282

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
徐闻县| 丰都县| 侯马市| 金阳县| 宿州市| 扶余县| 福鼎市| 山丹县| 怀来县| 花莲县| 赣榆县| 年辖:市辖区| 龙游县| 祁门县| 左云县| 烟台市| 调兵山市| 花垣县| 邢台县| 巩义市| 汉阴县| 新田县| 新野县| 蕉岭县| 方正县| 乌海市| 江城| 盖州市| 鸡东县| 班玛县| 大冶市| 当涂县| 柳州市| 海南省| 黄陵县| 扎囊县| 江油市| 卓尼县| 格尔木市| 县级市| 耿马|