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p482.6
p482.6
規(guī)格:
貨期:
編號(hào):B217091
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p482.6
商品貨號(hào) B217091
Designations p482.6
GenBank Number

X01179

Species Homo sapiens, human
Depositors RM Lawn
Applications
The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb.
Vector
Construct size (kb): 12.19999980926514
Insert
DNA: genomic
Insert lengths(kb): 9.600000381469727
Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) [F8C]
Alleles: F3, E1, E1, E2, F1, F2, A1, A2
Insert Size (kb) 9.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 2.6; HindIII--9.6, 2.6; PstI--3.5, 2.6, 2.3, 1.2, 0.8, 0.5, 0.3; BamHI--7.3, 4.6; AccI--5.2, 2.9, 1.4, 1.4, 0.45.
The insert is an EcoRI fragment of a portion of intron 22. The polymorphic XbaI site is included in the sequence recognized by the probe.
The non-intron 2 band (6.6 kb in a KpnI/XbaI double digest) appears to be more precisely 2 bands at 6.6 and 6.8 kb which are X-chromosomal in origin and may be polymorphic (5.4 + 1.4 and 5.2 + 1.4).
The 6.2 and 1.4 kb fragments are detected by hybridizing a 1.6 kb BstXI fragment to genomic DNA digested with XbaI and KpnI. This fragment seems superior in detecting the XbaI polymorphism.
The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb.
References

Lillicrap DP, et al. Variation of the non-factor VIII sequences detected by a probe from intron 22 of the factor VIII gene. Blood 75: 139-143, 1990. PubMed: 1967212

Taylor SA, et al. A BstXI polymorphism detected by the factor VIII genomic probe p482.6 (F8C). Nucleic Acids Res. 17: 6426, 1989. PubMed: 2570405

Suthers GK, et al. Physical mapping of new DNA probes near the fragile X mutation (FRAXA) by using a panel of cell lines. Am. J. Hum. Genet. 47: 187-195, 1990. PubMed: 2378346

Janco RL, et al. Improved efficacy of carrier assignment in hemophilia A using intragenic probes. Am. J. Hum. Genet. 39: A95, 1986.

Youssoufian H, et al. Characterization of five partial deletions of the factor VIII gene. Proc. Natl. Acad. Sci. USA 84: 3772-3776, 1987. PubMed: 3035554

Wion KL, et al. A new polymorphism in the factor VIII gene for prenatal diagnosis of hemophilia A. Nucleic Acids Res. 14: 4535-4542, 1986. PubMed: 3012474

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