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pK194
pK194
規(guī)格:
貨期:
編號(hào):B220498
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pK194
商品貨號(hào) B220498
Designations pK194
Depositors RK Holmes
Biosafety Level 1
Vector Information
Size (kb): 2.4319999217987060
Vector: pK194 (plasmid)
Promoters: Promoter lac
Construction: pK184 M13mp19
Marker(s):kanR
Construct size (kb): 2.431999921798706
Features: insert detection: lacZ'
marker(s): kanR
promoter: lac
replicon: p15A
terminator: none
Applications
A general purpose plasmid vector for use in complementation analysis with plasmids having a ColE1 or pMB1 origin of replication.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; HindIII--2.4; PstI--2.4; BamHI--2.4; XbaI--2.4.
A general purpose plasmid vector for use in complementation analysis with plasmids having a ColE1 or pMB1 origin of replication.
Constructed by M.G. Jobling.
pK184 (ATCC 37766) and pK194 (ATCC 37767) have the multiple cloning sites in opposite orientations, interrupting the lacZalpha coding sequence.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Jobling MG, Holmes RK. Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZalpha and pUC18 or pUC19 multiple cloning sites. Nucleic Acids Res. 18: 5315-5316, 1990. PubMed: 2402474

Randall K Holmes, personal communication

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