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Distribution host: Escherichia coli JM109

Size (kb): 5.8000001907348630

DESCRIPTION OF VECTOR:
Intact vector size: 5.800
Type of vector: plasmid
Cloning sites: SstI KpnI SmaI BamHI XbaI AatII mLuI NcoI BglII XhoI StuI
SphI HindIII
Polylinker sites: EcoRI SstI KpnI SmaI BamHI XbaI SalI AatII mLuI NcoI BglII
XhoI StuI PstI SphI HIndIII
Construction: pCB267, pQF40
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
insert detection: phoA
marker(s): ampR
promoter: none
replicon: pMB1, pRO1614 (1600)
terminator: T1

Vector: pQF60 (plasmid)

Promoters: Promoter none

Construction: pCB267, pQF40

Marker(s):ampR

Construct size (kb): 5.800000190734863

Features: insert detection: phoA
marker(s): ampR
promoter: none
replicon: pMB1, pRO1614 (1600)
terminator: T1

promoter-cloning vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): HindIII--5.8; BamHI--5.8; EcoRI--4.6, 1.2; XbaI--5.8.

Promoter-cloning shuttle vector permitting visual detection of activity.

Uses the promoterless alkaline phosphatase gene (phoA) as the reporter group.

Translational stop codons in all reading frames are located between the MCS and the translational initiation codon of the reporter gene.

The order of the major features in this plasmid is: ori(pMB1) - ori(1600) - ampR - terminator - multiple cloning site - phoA.

Temperature: 37.0°C