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<i>Clostridioides difficile</i> (Prevot) Lawson et al.
<i>Clostridioides difficile</i> (Prevot) Lawson et al.
規(guī)格:
貨期:
編號:B228538
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Clostridioides difficile (Prevot) Lawson et al.
商品貨號 B228538
Deposited As Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations 545
Isolation
Feces of patient with pseudomembranous colitis, Belgium.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties Serogroup C
Product Format frozen
Shipping Information Frozen
Preceptrol&reg; no
Genotype Toxinotype: 0
Type Strain no
Comments
Preparation of Clostridium difficile strain 545 ATCC® 43596-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Toxinotype 0
Ribotype 012
Presence of tcdA and tcdB genes confirmed by PCR.
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Binary toxin gene cdtB not amplified by PCR
Medium ATCC® Medium 2107: Modified Reinforced Clostridial
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor M Delmee
References

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

Kink JA, Williams JA. Antibodies to recombinant Clostridium difficile toxins A and B are an effective treatment and prevent relapse of C. difficile-associated disease in a hamster model of infection. Infect. Immun. 66: 2018-2025, 1998. PubMed: 9573084

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

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