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pB8 [lambdaB1]
pB8 [lambdaB1]
規(guī)格:
貨期:
編號(hào):B231590
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pB8 [lambdaB1]
商品貨號(hào) B231590
Designations pB8 [lambdaB1]
GenBank Number

J02775

Species Homo sapiens, human
Depositors JL Breslow
Vector
Construct size (kb): 4.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.600000023841858
Tissue: hepatocellular carcinoma Hep G2 cell line
Gene product: apolipoprotein B (including Ag(x) antigen) [APOB]
Alleles: B1, B3, B5, D2, B2, B4, D1
Insert Size (kb) 1.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.2, EcoRI/PstI--2.7, 1.6; EcoRI--4.2; HindIII--3.4, 0.7 doublet; PvuII--2.3, 1.3, 0.67.
Detects the hypervariable VNTR RFLP of the AT-rich minisatellite at the 3' end of APOB when genomic DNA is digested with HindIII and MspI but not with XbaI.
Insert ends are from linkers and are not present in the cDNA itself.
References

Hegele RA, et al. Apolipoprotein B-gene DNA polymorphisms associated with myocardial infarction. N. Engl. J. Med. 315: 1509-1515, 1986. PubMed: 3024002

Huang LS, et al. Human apolipoprotein B cDNA clone isolation and demonstration that liver apolipoprotein B mRNA is 22 kilobases in length. Proc. Natl. Acad. Sci. USA 82: 6825-6829, 1985. PubMed: 2995989

Huang LS, Breslow JL. A unique AT-rich hypervariable minisatellite 3' to the ApoB gene defines a high information restriction fragment length polymorphism. J. Biol. Chem. 262: 8952-8955, 1987. PubMed: 2885324

Jan L Breslow, personal communication

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