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Construct size (kb): 3.269999980926514

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR1000
Intact vector size: 3.000
Type of vector: plasmid
Vector end: T overhangs
Vector end: T overhangs
Cloning sites:
Polylinker sites: ApaI KpnI SacI SpeI PacI HindIII XmaI ins site NotI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): kanR
promoter: lac, T7
replicon: colE1
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HLA-DQB1
Other gene symbols: HLA-DQB
Genomic copy number: unique
Gene name: major histocompatibility complex, class II, DQ beta 1
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 p21.3
Tissue: peripheral blood leukocytes
Type of DNA: genomic
Insert end: Modification: A overhangs
Insert end: Modification: A overhangs
Insert size (kb): 0.27
Cross references: DNA Seq. Acc.: M87041

Insert lengths(kb): 0.2700000107288361

Tissue: peripheral blood leukocytes

Gene product: major histocompatibility complex, class II, DQ beta 1(major histocompatibility complex, class II, lymphocyte antigen DQB) [HLA-DQB]

Restriction digests of the clone give the following sizes (kb): BglI--2.9, 0.38; SmaI--2.8, 0.4, 0.2; BamHI--uncut; EcoRI/HindIII--3.0, 0.32; HindIII--3.3.

The insert contains the following restriction sites (bp from the 5' end): BglI--129; SmaI--188.

Encodes the *0607 or the *06BRI1 allele, which differs from DQB1*0603 by a substitution at codon 57 [GTT (valine) replacing GAT (aspartic acid)].